- PII
- S0555109925010075-1
- DOI
- 10.31857/S0555109925010075
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 61 / Issue number 1
- Pages
- 68-76
- Abstract
- A new method is proposed for the early diagnosis of the causal agent of grapes downy mildew, Plasmopara viticola, based on the method of quantitative real-time PCR (qPCR RT) using SYBR Green I fluorescence. Six pairs primers were developed for the diagnosis of P. viticola, among the designed primers, PvITS1_2-real-s/a demonstrated the highest effectiveness for early detection of grapevine downy mildew with a strong positive correlation with the metagenomic data of P. viticola distribution in Far Eastern grape species and varieties, where a linear dependence was found (R2 = 0.86). Thus, qPCR RT of PvITS1_2 can be used for early detection and monitoring of asymptomatic P. viticola infections. The developed method can be used as a basis for predicting epidemics of downy mildew of grapes and for its control in vineyards.
- Keywords
- стратегии управления заболеваниями растений милдью Vitis amurensis NGS
- Date of publication
- 12.09.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 15
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