- PII
- S3034574XS0555109925020031-1
- DOI
- 10.7868/S3034574X25020031
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 61 / Issue number 2
- Pages
- 139-148
- Abstract
- Using directly engineered derivatives of previously constructed adipate-producing Escherichia coli MG1655 lacIQ, ∆ackA-pta, ∆poxB, ∆ldhA, ∆adhE, PL-SDϕ10-atoB, Ptrc-ideal-4-SDϕ10-fadB, ∆fadE, PL-SDϕ10-tesB, ∆yciA, Ptrc-ideal-4-SDϕ10-fabI, PL-SDϕ10-paaJ, ∆aceBAKϕ, ∆glcB и MG1655 lacIQ, ∆ackA-pta, ∆poxB, ∆ldhA, ∆adhE, PL-SDϕ 10-atoB Ptrc-ideal-4-SD ϕ10-fadB, PL-SDϕ10-tesB, ∆yciA, Ptrc-ideal-4-SDϕ10-fadE, PL-SDϕ10-paaJ, ∆aceBAK, ∆glcB the feasibility of suberic acid biosynthesis from glucose by this bacterium resulting from the reversal of the native fatty acid β-oxidation pathway was demonstrated. The condensation of acetyl-CoA with succinyl-CoA and adipyl-CoA was ensured in recombinants by 3-oxoadipyl-CoA thiolase PaaJ, whereas the putative acetyl-CoA C-acetyltransferase YqeF was unable to catalyse the respective reactions. The biosynthesis of ~60 µM suberic acid was achieved upon significant enhancement in the strains of the expression of the bifunctional (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA reductase gene, fadB. Subsequent inactivation of succinate dehydrogenase in the strains increased the intracellular availability of succinyl-CoA for the initiation of the first round of cycle reversal and favored an increase in the accumulation of the target compound by the recombinants to ~75 µM. The results provide a framework for the development of highly efficient producing strains for bio-based production of suberic acid from renewable raw materials.
- Keywords
- β-окисление жирных кислот метаболическая инженерия субериновая кислота тиолаза Escherichia coli
- Date of publication
- 12.11.2024
- Year of publication
- 2024
- Number of purchasers
- 0
- Views
- 22
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